Mechanism-based toxicology.

A review of acrylamide toxicity and its mechanism

Acrylamide (AA) is an important industrial chemical agent that is mainly used in the production of polymers and copolymers. Recently it has been attention because of its production in the diet at high-temperature (>120 ºC) processes such as cooking, frying, toasting, roasting or baking of high carbohydrate foods. According to high exposure to acrylamide, recognition of its toxic effect is ...

Food for Thought ... Mechanistic Validation

this series of articles offers perspectives on areas requiring change, mainly in (regulatory) toxicology such as the assessment of chemicals (Hartung, 2010c), cosmetics (Hartung, 2008b), food (Hartung and Koëter, 2008), medical countermeasures (Hartung and Zurlo, 2012), nanoparticles (Hartung, 2010b), and earlier drugs (Hartung, 2001), as well as basic research (Gruber and Hartung, 2004). the s...

Proteomics for systems toxicology

Current toxicology studies frequently lack measurements at molecular resolution to enable a more mechanism-based and predictive toxicological assessment. Recently, a systems toxicology assessment framework has been proposed, which combines conventional toxicological assessment strategies with system-wide measurement methods and computational analysis approaches from the field of systems biology...

Accelerated Cytotoxicity Mechanism Screening

By discovering how chemical compounds/xenobiotics cytotoxicity is affected when their metabolic pathways are inhibited or activated, the metabolic pathways that activate versus detoxify chemical compound can be identified. Reactive metabolites contributing to cytotoxicity can also be identified. In this lecture, the pretreatment of inhibitors and activators of xenobiotic metabolizing enzymes as...

Mouse or molecule? Mechanism-based toxicology in cancer risk assessment.

Toxicity Evaluation of 6-Mercaptopurine Using Accelerated Cytotoxicity Mechanism Screening (ACMS) techniques

6- Mercaptopurine (6-MP) is widely used in clinic as an immunosuppressive for treatment of acute lymphocytic leukemia, Crohn's disease, and ulcerative colitis with documented unpredictable hepatotoxicity. The potential molecular cytotoxic mechanisms of 6-MP against isolated rat hepatocytes were searched in this study using “Accelerated Cytotoxicity Mechanism Screening (ACMS)” techniques. The co...